The epIgG project, is a large cross-disciplinary collaboration of five labs from Sweden and Switzerland that is funded by the Knut & Alice Wallenberg Foundation. The overall epIgG project aims to explain the absolute link between IgG antibody epitope and their function at the protein-protein interface of host-bacteria interactions
The project aims to develop new integrin biosensors, building upon the principle shown in our paper “Coordinated integrin activation by actin-dependent force during T-cell migration” in Nature Communications.
The sysmic project focuses on cell migration, a complex cellular process highly relevant to cancer. It is a SSF-funded grant within their Systems Biology program, led by Staffan Strömblad at Karolinska with the Nordenfelt Lab as one of the main participants.
We will further develop and apply a systems microscopy strategy by combining recently developed quantitative imaging and statistical modeling with a number of new developing imaging tools and, in a novel manner, with emerging cellular omics. All tailored to systematically unravel cellular and molecular dynamics of cell migration.
Molecular basis for phagocytosis
The project aims to quantitatively determine the molecular requirements for Fc-mediated and CR-mediated phagocytosis.
Bacterial invasion of human cells
The project aims to find common pathways used by multiple bacterial pathogens to invade human cells. The precise timing and molecular stoichometry will be studied.
Antibody binding model
The project aims to establish a biophysical model for the binding of IgG antibodies to bacterial surfaces. This is a development of our paper “Antibody orientation at bacterial surfaces is related to invasive infection” in Journal of Experimental Medicine.
Quantification of neutrophil extracellular traps
The project aims to develop software for automatic quantification of NETs.
Together with Prof Jonas Tegenfeldt Ass Prof Vinay Swaminathan, and Assoc Prof Chris Madsen, the project will test the relationship between the bio-mechanical phenotypes of cancer cell populations, cell lines as well as metastasized circulating tumor cells (CTCs) the fundamental cellular property of directed cell migration and investigate the molecular mechanisms that govern these properties. Unique technological advances from microfluidics and advanced optical microscopy, including super resolution microscopy will be combined to shed new light on a central disease mechanism, and in the end open up for novel diagnostics and more precise treatments of cancer.
Morphology and virulence among bacteria
Bacterial morphology has been used for long time to classify bacterial species and strains. However, it has not been investigated to any great extent what benefits different morphologies confer to the bacteria or what challenges they may face for certain morphologies. In a collaboration between Physics (Prof Jonas Tegenfeldt) and Medicine the project will study the effect of morphology of bacteria and bacterial clusters by advanced microfluidics for sorting of the bacteria as a function of their physical properties, followed by detailed investigations of their interaction with the human cell lines as a function of their properties. The aim is to gain knowledge how the physical properties of the bacteria affect their interaction with the host.